The fresh matchmaking between details from genetic (P
Metropolises of Platanthera chlorantha (PS1 and you will PS2, PB1–PB4, circles) and you can Cephalanthera rubra (CK1 and CK2, CB1–CB7, triangles) communities inside northern-east Poland.
Studies town and you will testing
We examined half a dozen P. chlorantha and you may 9 C. rubra populations when you look at the north-eastern Poland (Bialowieza and you may Knyszynska Primeval Tree, Szeszupa lake valley) inside the pure, semi-sheer and you may anthropogenic organizations out-of national and you may land areas, supplies and protected areas, for example Natura 2000 sites ( Fig. 1). The actual fact that he is based in safe section, of many occur to your rail embankments, together ways and you will pathways within the forests or even in clearings.
The latest testing techniques depended to the society dimensions. Leaf examples out-of nearly all ramets in this communities of every kinds was in fact pulled (but inhabitants PS2; Desk step one); no samples was in fact taken from busted otherwise really younger anyone. One hundred and you can ninety-seven samples from P. chlorantha and 95 examples out-of C. rubra was indeed accumulated. Leaf tissues are maintained freeze until it could be kept during the ?80 °C, pending allozyme study. All collected products were utilized to own allozyme study. Leer más